Evaluation of Etest and macrodilution broth method for antifungal susceptibility testing of Candida sp strains isolated from oral cavities of AIDS patients
Rev. Inst. Med. Trop. Säo Paulo
;
44(3): 121-125, 2002. tab
Article
in English
| LILACS
| ID: lil-314538
RESUMO
A comparison of the Etest and the reference broth macrodilution susceptibility test for fluconazole, ketoconazole, itraconazole and amphotericin B was performed with 59 of Candida species isolated from the oral cavities of AIDS patients. The Etest method was performed according to the manufacturer's instructions, and the reference method was performed according to National Committee for Clinical Laboratory Standards document M27-A guidelines. Our data showed that there was a good correlation between the MICs obtained by the Etest and broth dilution methods. When only the MIC results at ± 2 dilutions for both methods were considered, the agreement rates were 90.4 percent for itraconazole, ketoconazole and amphotericin B and 84.6 percent for fluconazole of the C. albicans tested. In contrast, to the reference method, the Etest method classified as susceptible three fluconazole-resistant isolates and one itraconazole-resistant isolate, representing four very major errors. These results indicate that Etest could be considered useful for antifungal sensitivity evaluation of yeasts in clinical laboratories
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Candida
/
Microbial Sensitivity Tests
/
Antifungal Agents
Type of study:
Practice guideline
Limits:
Humans
Language:
English
Journal:
Rev. Inst. Med. Trop. Säo Paulo
Journal subject:
Tropical Medicine
Year:
2002
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Goiás/BR
/
Universidade de Säo Paulo/BR
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