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Experimental model of Candida Albicans (serotypes A and B) adherence In Vitro
Pires, Maria de Fátima Costa; Corrêa, Benedito; Gambale, Walderez; Paula, Claudete Rodrigues.
  • Pires, Maria de Fátima Costa; Instituto Adolfo Lutz. Seçäo de Microscopia Eletrônica. Säo Paulo. BR
  • Corrêa, Benedito; Universidade de Säo Paulo, Säo Paulo, SP, Brasil. Instituto de Ciências Biomédicas. Departamento de Microbiologia. Säo Paulo. BR
  • Gambale, Walderez; Universidade de Säo Paulo, Säo Paulo. Instituto de Ciências Biomédicas. Departamento de Microbiologia. Säo Paulo. BR
  • Paula, Claudete Rodrigues; Universidade de Säo Paulo. Instituto de Ciências Biomédicas. Departamento de Microbiologia. Säo Paulo. BR
Braz. j. microbiol ; 32(3): 163-169, July-Sept., 2001. ilus, tab
Article in English | LILACS | ID: lil-316963
RESUMO
The adherence of Candida albicans is considered an important virulence factor since it determines the colonization of the yeast in various regions of the host, being the initial cause of the infection. In this work we studied the adherence of C. albicans (serotypes A and B) to HeLa and Vero cells after culture for 18 h and 30 h in chemically defined medium (YNB) plus carbohydrate sources such as glucose, sucrose, maltose, galactose and mannose (500 mM). Each assay was performed in triplicate. The best results were obtained with the medium containing glucose. The adherence was greatest in 18 h cultures at 37§C and at 1 h contact time. The interaction of yeast cells with the host cells was studied by scanning microscopy.
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Full text: Available Index: LILACS (Americas) Main subject: Yeasts / In Vitro Techniques / Candida albicans / Candidiasis Type of study: Prognostic study Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2001 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Adolfo Lutz/BR / Universidade de Säo Paulo/BR / Universidade de Säo Paulo, Säo Paulo/BR / Universidade de Säo Paulo, Säo Paulo, SP, Brasil/BR

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Full text: Available Index: LILACS (Americas) Main subject: Yeasts / In Vitro Techniques / Candida albicans / Candidiasis Type of study: Prognostic study Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2001 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Adolfo Lutz/BR / Universidade de Säo Paulo/BR / Universidade de Säo Paulo, Säo Paulo/BR / Universidade de Säo Paulo, Säo Paulo, SP, Brasil/BR