An in vitro system from Plasmodium falciparum active in endogenous mRNA translation
Mem. Inst. Oswaldo Cruz
;
95(2): 231-235, Mar.-Apr. 2000.
Article
in English
| LILACS
| ID: lil-319971
ABSTRACT
An in vitro translation system has been prepared from Plasmodium falciparum by saponin lysis of infected-erythrocytes to free parasites which were homogeneized with glass beads, centrifuged to obtain a S-30 fraction followed by Sephadex G-25 gel filtration. This treatment produced a system with very low contamination of host proteins (<1). The system, optimized for Mg2+ and K+, translates endogenous mRNA and is active for 80 min which suggests that their protein factors and mRNA are quite stable.
Full text:
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Index:
LILACS (Americas)
Main subject:
Plasmodium falciparum
/
Protein Biosynthesis
/
RNA, Messenger
/
RNA, Protozoan
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2000
Type:
Article
Affiliation country:
Venezuela
Institution/Affiliation country:
Universidad de Carabobo-Nucleo Aragua/VE
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