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Immunogenicity test of tetanus component in adsorbed vaccines by toxin binding inhibition test
Matos, Denise Cristina Souza; Marcovistz, Rugimar; Cabello, Pedro Hernan; Georgini, Ricardo Amaral; Sakauchi, Dirce; Silva, Luciana Leite da.
  • Matos, Denise Cristina Souza; Instituto Butantan. Serviço de Controle de Qualidade. Säo Paulo. BR
  • Marcovistz, Rugimar; Instituto Butantan. Serviço de Controle de Qualidade. Säo Paulo. BR
  • Cabello, Pedro Hernan; Fiocruz. Departamento de Genética. Rio de Janeiro. BR
  • Georgini, Ricardo Amaral; Instituto Butantan. Serviço de Controle de Qualidade. Säo Paulo. BR
  • Sakauchi, Dirce; Instituto Butantan. Serviço de Controle de Qualidade. Säo Paulo. BR
  • Silva, Luciana Leite da; Instituto Butantan. Serviço de Controle de Qualidade. Säo Paulo. BR
Mem. Inst. Oswaldo Cruz ; 97(6): 909-913, Sept. 2002. tab, graf
Article in English | LILACS, SES-SP | ID: lil-320153
ABSTRACT
Samples from 20 lots of diphtheria-tetanus (adult use dT) vaccine and from 20 lots of diphtheria-tetanus-pertussis (DTP) vaccine were used to standardize and validate the in vitro toxin binding inhibition (ToBI) test for the immunogenicity test of the tetanus component. The levels of tetanus antitoxin obtained by ToBI test were compared to those obtained using the toxin neutralization (TN) test in mice routinely employed to perform the quality control of the tetanus component in adsorbed vaccines. The results ranged from 1.8 to 3.5 IU/ml for dT and 2 to 4 IU/ml for DTP by ToBI test and 1.4 to 3 IU/ml for dT and 1.8 to 3.5 IU/ml for DTP by TN in mice. These results were significantly correlated. From this study, it is concluded that the ToBI test is an alternative to the in vivo neutralization procedure in the immunogenicity test of the tetanus component in adsorbed vaccines. A substantial refinement and a reduction in use of animals can be achieved
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Full text: Available Index: LILACS (Americas) Main subject: Tetanus Antitoxin / Diphtheria-Tetanus-Pertussis Vaccine / Diphtheria-Tetanus Vaccine Limits: Animals Language: English Journal: Mem. Inst. Oswaldo Cruz Year: 2002 Type: Article / Project document Institution/Affiliation country: Fiocruz/BR / Instituto Butantan/BR

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Full text: Available Index: LILACS (Americas) Main subject: Tetanus Antitoxin / Diphtheria-Tetanus-Pertussis Vaccine / Diphtheria-Tetanus Vaccine Limits: Animals Language: English Journal: Mem. Inst. Oswaldo Cruz Year: 2002 Type: Article / Project document Institution/Affiliation country: Fiocruz/BR / Instituto Butantan/BR