Your browser doesn't support javascript.
loading
Standardization of dot-ELISA for the serological diagnosis of toxocariasis and comparison of the assay with ELISA
Camargo, Eide Dias; Nakamura, Paulo Mutuko; Vaz, Adelaide José; Silva, Marcos Vinicius da; Chieffi, Pedro Paulo; Melo, Elisabete Ourique de.
  • Camargo, Eide Dias; Instituto Adolfo Lutz. São Paulo. BR
  • Nakamura, Paulo Mutuko; Instituto Adolfo Lutz. São Paulo. BR
  • Vaz, Adelaide José; Instituto Adolfo Lutz. São Paulo. BR
  • Silva, Marcos Vinicius da; Instituto Adolfo Lutz. São Paulo. BR
  • Chieffi, Pedro Paulo; Instituto de Medicina Tropical. São Paulo. BR
  • Melo, Elisabete Ourique de; Instituto de Medicina Tropical. São Paulo. BR
Rev. Inst. Med. Trop. Säo Paulo ; 34(1): 55-60, Jan.-Feb. 1992.
Article in English | LILACS | ID: lil-320629
RESUMO
The dot-enzyme-linked immunosorbent assay (dot-ELISA) was standardized using somatic (S) and excretory-secretory (ES) antigens of Toxocara-canis for the detection of specific antibodies in 22 serum samples from children aged 1 to 15 years, with clinical signs of toxocariasis. Fourteen serum samples from apparently normal individuals and 28 sera from patients with other pathologies were used as controls. All samples were used before and after absorption with Ascaris suum extract. When the results were evaluated in comparison with ELISA, the two tests were found to have similar sensitivity, but dot-ELISA was found to be more specific in the presence of two antigens studied. Dot-ELISA proved to be effective for the diagnosis of human toxocariasis, presenting advantages in terms of yield, stability, time and ease of execution and low cost.
Subject(s)
Fulltext
Print
XML
Search on Google
Full text: Available Index: LILACS (Americas) Main subject: Toxocariasis Type of study: Diagnostic study Limits: Adolescent / Child / Child, preschool / Female / Humans / Infant / Male Language: English Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 1992 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Adolfo Lutz/BR / Instituto de Medicina Tropical/BR

Similar

MEDLINE
LILACS
LIS
Fulltext
Print
XML
Search on Google
Full text: Available Index: LILACS (Americas) Main subject: Toxocariasis Type of study: Diagnostic study Limits: Adolescent / Child / Child, preschool / Female / Humans / Infant / Male Language: English Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 1992 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Adolfo Lutz/BR / Instituto de Medicina Tropical/BR