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UltramicroELISA indirecto para la detección de anticuerpos totales a Citomegalovirus en suero humano / Indirect ultramicroElisa for the detection of total antibodies against cytomegaloviruses in human blood
Laferte, José; Marrero, Miguel; Alvarez, Maritza; Jomarron, Liliana; Garcia, Serafina; Vazquez, Susana; Morier, Luis; Ulacia, Mário; Melchor, Antonio.
  • Laferte, José; Instituto de Medicina Tropical Pedro Kouri Siboney. Departamento de Virologia. La Habana. CU
  • Marrero, Miguel; Instituto de Medicina Tropical Pedro Kouri Siboney. Departamento de Virologia. La Habana. CU
  • Alvarez, Maritza; Instituto de Medicina Tropical Pedro Kouri Siboney. Departamento de Virologia. La Habana. CU
  • Jomarron, Liliana; Instituto de Medicina Tropical Pedro Kouri Siboney. Departamento de Virologia. La Habana. CU
  • Garcia, Serafina; Instituto de Medicina Tropical Pedro Kouri Siboney. Departamento de Virologia. La Habana. CU
  • Vazquez, Susana; Instituto de Medicina Tropical Pedro Kouri Siboney. Departamento de Virologia. La Habana. CU
  • Morier, Luis; Instituto de Medicina Tropical Pedro Kouri Siboney. Departamento de Virologia. La Habana. CU
  • Ulacia, Mário; Banco de Sangue Provincial. La Habana. CU
  • Melchor, Antonio; Centro de Inmunoensayo. La Habana. CU
Rev. Inst. Med. Trop. Säo Paulo ; 34(1): 43-47, Jan.-Feb. 1992.
Article in Spanish | LILACS | ID: lil-320631
RESUMO
We have standardized an indirect ultramicro ELISA assay for detecting antibodies to human Cytomegalovirus (CMV) using human serum samples (UMELISA CMV). The optimal concentration of coating antigen (30 ug/ml), serum dilution (140) and anti-human conjugate working dilution (11500), were determined by a check board titration method. The UMELISA CMV was compared with the latex agglutination test for antibodies to CMV (Dupont de Nemours) and with an indirect immunofluorescent method. The results have showed the high coincidence, sensitivity and especificity of the proposed assay regarding the two methods compared with, and supporting its use either for a blood donors screening or in the serological diagnosis of this infection by paired serum samples.
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Full text: Available Index: LILACS (Americas) Main subject: Enzyme-Linked Immunosorbent Assay / Cytomegalovirus / Antibodies, Viral Type of study: Diagnostic study Limits: Humans Language: Spanish Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 1992 Type: Article Affiliation country: Cuba Institution/Affiliation country: Banco de Sangue Provincial/CU / Centro de Inmunoensayo/CU / Instituto de Medicina Tropical Pedro Kouri Siboney/CU

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Full text: Available Index: LILACS (Americas) Main subject: Enzyme-Linked Immunosorbent Assay / Cytomegalovirus / Antibodies, Viral Type of study: Diagnostic study Limits: Humans Language: Spanish Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 1992 Type: Article Affiliation country: Cuba Institution/Affiliation country: Banco de Sangue Provincial/CU / Centro de Inmunoensayo/CU / Instituto de Medicina Tropical Pedro Kouri Siboney/CU