Identification of Toxocara canis antigens by Western blot in experimentally infected rabbits
Rev. Inst. Med. Trop. Säo Paulo
;
44(4): 213-216, July-Aug. 2002. ilus, graf
Article
in English
| LILACS
| ID: lil-321223
RESUMO
Toxocariasis is a frequent helminthiasis that can cause visceral and ocular damage in humans specially in children. The identification of specific antigens of Toxocara canis is important in order to develop better diagnostic techniques. Ten rabbits were infected orally with a dose of 5000 Toxocara canis embryonated eggs. Rabbits were bled periodically and an ELISA assay was performed to determine levels of specific Toxocara IgG antibodies. ELISA detected antibodies at day 15 after infection. Western blot (WB) assay was performed using excretory/secretory antigens (E/S) of T. canis second stage larvae. Different antigen concentrations were evaluated: 150, 200, 250 and 300 æg/mL. The concentration of 250 æg/mL was retained for analysis. Rabbit sera were diluted 1:100. Secondary antibody was used at a dilution of 1:1000. Results of WB indicated that in the first month after infection specific antibodies against the 200 KDa, 116 KDa, 92 KDa and 35 KDa antigens were detected; antibodies against the 92 KDa, 80 KDa, 66 KDa, 45 KDa, 31 KDa and 28 KDa antigens appeared later. All positive sera in the ELISA test were also positive in WB. Two antigen bands, 92 KDa and 35 KDa, were identified since the beginning and throughout the course of infection. These antigens merit further evaluation as candidates for use in diagnosis
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Immunoglobulin G
/
Antibodies, Helminth
/
Blotting, Western
/
Toxocara canis
/
Antigens, Helminth
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Rev. Inst. Med. Trop. Säo Paulo
Journal subject:
Tropical Medicine
Year:
2002
Type:
Article
Affiliation country:
Colombia
Institution/Affiliation country:
Universidad Nacional/CO
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