Inmunodiagnóstico de la triquinosis humana / Inmunodiagnosis of human trichinosis
Bol. chil. parasitol
;
57(3/4): 47-53, jul. 2001. tab
Article
in Spanish
| LILACS
| ID: lil-321447
RESUMO
An indirect hemagglutination test (IHAT) and an ELISA test for trichinosis using as antigen a larvae soluble fraction from Trichinella spiralis was carried out for the detection of IgG, IgM and IgA specific antibodies in 113 serum samples from patients confirmed or suspected to have trichinosis by strong clinical and epidemiological evidences (Group 1). The same test were also performed on 110 serum samples corresponding to patients without strong enidences of having trichinosis (Group II). In Group 1 the correponding sensitivities for RHAI, ELISA IgG, ELISA IgM, ELISA IgA were: 82.3-85.8-88.5 and 88.5 percent respectively. Seventeen patients were tested again a week after the first analysis (10 of them corresponded to negative ones), increasing the positivity: 23.5-100.0;35.3-100.0;41.2-100.0 and 41.2-100.0 percent for RHAI, ELISA IgG, ELISA IgM and ELISA IgA, respectively. Other two patients were followed-up for 5 years. IHAT and ELISA IgG remained positive, whereas ELISA IgM and ELISA IgA were constantly negative betweeen 17 and 32 months in one case, and between 48 and 60 months in the other (this last one had presented a severe clinic disease). In the group II, four patients were positive with IHAT, however only one for ELISA IgA, the latter also presented ELISA IgM near the cut off. The use of ELISA IgG, ELISA IgM and ELISA IgA in the immunodiagnosis of trichinosis is discussed
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Trichinellosis
/
Trichinella spiralis
Type of study:
Diagnostic study
Limits:
Adolescent
/
Adult
/
Child, preschool
/
Female
/
Humans
/
Male
Language:
Spanish
Journal:
Bol. chil. parasitol
Journal subject:
ALIMENTOS
/
Parasitology
Year:
2001
Type:
Article
Affiliation country:
Chile
Institution/Affiliation country:
Universidad de Chile/CL
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