Non-selective cation channels and oxidative stress-induced cell swelling
Biol. Res
;
35(2): 215-222, 2002. graf
Article
in English
| LILACS
| ID: lil-323344
RESUMO
Necrosis is considered as a non-specific form of cell death that induces tissue inflammation and is preceded by cell swelling. This increase in cell volume has been ascribed mainly to defective outward pumping of Na+ caused by metabolic depletion and/or to increased Na+ influx via membrane transporters. A specific mechanism of swelling and necrosis driven by the influx of Na+ through nonselective cation channels has been recently proposed (Barros et al., 2001a). We have characterized further the properties of the nonselective cation channel (NSCC) in HTC cells. The NSCC shows a conductance of approximately 18 pS, is equally permeable to Na+ and K+, impermeant to Ca2+, requires high intracellular Ca2+ as well as low intracellular ATP for activation and is inhibited by flufenamic acid. Hydrogen peroxide induced a significant increase in cell volume that was dependent on external Na+. We propose that the NSCC, which is ubiquitous though largely inactive in healthy cells, becomes activated under severe oxidative stress. The ensuing Na+ influx initiates via positive feedback a series of metabolic and electrolytic disturbances, resulting in cell death by necrosis
Full text:
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Index:
LILACS (Americas)
Main subject:
Carcinoma, Hepatocellular
/
Oxidative Stress
/
Ion Channels
/
Liver Neoplasms
Limits:
Animals
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2002
Type:
Article
/
Project document
Affiliation country:
Chile
Institution/Affiliation country:
Universidad de Chile/CL
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