Genetic markers between Biomphalaria glabrata snails susceptible and resistant to Schistosoma mansoni infection
Mem. Inst. Oswaldo Cruz
;
97(suppl.1): 53-58, Oct. 2002. ilus, tab
Article
in English
| LILACS
| ID: lil-325024
ABSTRACT
The analysis of the genetic variability related to susceptibility to Schistosoma mansoni infection in the vector of the genus Biomphalaria is important in terms of a better understanding of the epidemiology of schistosomiasis itself, the possible pathological implications of this interaction in vertebrate hosts, and the formulation of new strategies and approaches for disease control. In the present study, the genetic variability of B. glabrata strains found to be resistant or susceptible to S. mansoni infection was investigated using DNA amplification by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). The amplification products were analyzed on 8 percent polyacrylamide gel and stained with silver. We selected 10 primers, since they have previously been useful to detect polymorphism among B. glabrata and/or B. tenagophila. The results showed polymorphisms with 5 primers. Polymorphic bands observed only in the susceptible strain. The RAPD-PCR methodology represents an adequate approach for the analysis of genetic polymorphisms. The understanding of the genetic polymorphisms associated to resistance may contribute to the future identification of genomic sequences related to the resistance/susceptibility of Biomphalaria to the larval forms of S. mansoni and to the development of new strategies for the control of schistosomiasis
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Schistosoma mansoni
/
Biomphalaria
/
Random Amplified Polymorphic DNA Technique
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2002
Type:
Article
/
Congress and conference
Affiliation country:
Brazil
/
Egypt
Institution/Affiliation country:
National Research Center/EG
/
Universidade Estadual Paulista/BR
/
Universidade Estadual de Campinas/BR
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