Identification of snails within the Bulinus africanus group from East Africa by multiplex SNaPshotTManalysis of single nucleotide polymorphisms within the cytochrome oxidase subunit I
Mem. Inst. Oswaldo Cruz
;
97(suppl.1): 31-36, Oct. 2002. ilus, tab, graf
Article
in English
| LILACS
| ID: lil-325025
RESUMO
Identification of populations of Bulinus nasutus and B. globosus from East Africa is unreliable using characters of the shell. In this paper, a molecular method of identification is presented for each species based on DNA sequence variation within the mitochondrial cytochrome oxidase subunit I (COI) as detected by a novel multiplexed SNaPshotTM assay. In total, snails from 7 localities from coastal Kenya were typed using this assay and variation within shell morphology was compared to reference material from Zanzibar. Four locations were found to contain B. nasutus and 2 locations were found to contain B. globosus. A mixed population containing both B. nasutus and B. globosus was found at Kinango. Morphometric variation between samples was considerable and UPGMA cluster analysis failed to differentiate species. The multiplex SNaPshotTM assay is an important development for more precise methods of identification of B. africanus group snails. The assay could be further broadened for identification of other snail intermediate host species
Full text:
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Index:
LILACS (Americas)
Main subject:
Schistosomiasis haematobia
/
Bulinus
/
Electron Transport Complex IV
/
Polymorphism, Single Nucleotide
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2002
Type:
Article
/
Congress and conference
Affiliation country:
Canada
/
Denmark
/
United kingdom
Institution/Affiliation country:
Danish Bilharziasis Laboratory/DK
/
McGill University/CA
/
The Natural History Museum/GB
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