Characterization of Sm14 related components in different helminths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis
Mem. Inst. Oswaldo Cruz
;
97(suppl.1): 115-116, Oct. 2002. ilus
Article
in English
| LILACS
| ID: lil-325028
ABSTRACT
Sm14 was the first fatty acid-binding protein homologue identified in helminths. Thereafter, members of the same family were identified in several helminth species, with high aminoacid sequence homology between them. In addition, immune crossprotection was also reported against Fasciola hepatica infection, in animals previously immunized with the Schistosoma mansoni vaccine candidate, r-Sm14. In the present study, data on preliminary sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis of nine different helminth extracts focusing the identification of Sm14 related proteins, is reported. Out of these, three extracts - Ascaris suum (males and females), Echinostoma paraensei, and Taenia saginata - presented components that comigrated with Sm14 in SDS-PAGE, and that were recognized by anti-rSm14 policlonal serum, in Western blotting tests
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Schistosoma mansoni
/
Schistosomiasis mansoni
/
Helminth Proteins
/
Blotting, Western
/
Electrophoresis, Polyacrylamide Gel
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2002
Type:
Article
/
Congress and conference
Affiliation country:
Brazil
Institution/Affiliation country:
Fiocruz/BR
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