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Polymerase chain reaction and restriction fragment length polymorphism of cytocrome oxidase subunit I used for differentiation of Brazilian Biomphalaria species intermediate host of Schistosoma mansoni
Vidigal, Teofânia H. D. A; Montresor, Lângia C; Simpson, Andrew J. G; Carvalho, Omar S.
  • Vidigal, Teofânia H. D. A; Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Zoologia. Belo Horizonte. BR
  • Montresor, Lângia C; Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Zoologia. Belo Horizonte. BR
  • Simpson, Andrew J. G; Instituto Ludwig de Pesquisa sobre o Câncer. Säo Paulo. BR
  • Carvalho, Omar S; Fiocruz. Centro de Pesquisas René Rachou. Laboratório de Helmintoses Intestinais. Belo Horizonte. BR
Mem. Inst. Oswaldo Cruz ; 97(suppl.1): 47-52, Oct. 2002. ilus, tab
Article in English | LILACS | ID: lil-325031
ABSTRACT
The intermediate hosts of Schistosoma mansoni, in Brazil, Biomphalaria glabrata, B. tenagophila and B. straminea, were identified by restriction fragment length polymorphism analysis of the mitochondrial gene cytochrome oxidase I (COI). We performed digestions with two enzymes (AluI and RsaI), previously selected, based on sequences available in Genbank. The profiles obtained with RsaI showed to be the most informative once they were polymorphic patterns, corroborating with much morphological data. In addition, we performed COI digestion of B. straminea snails from Uruguay and Argentina
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Full text: Available Index: LILACS (Americas) Main subject: Schistosoma mansoni / Biomphalaria / Polymorphism, Restriction Fragment Length / RNA, Ribosomal / Polymerase Chain Reaction / Electron Transport Complex IV Limits: Animals Country/Region as subject: South America / Argentina / Brazil / Uruguay Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2002 Type: Article / Congress and conference Affiliation country: Brazil Institution/Affiliation country: Fiocruz/BR / Instituto Ludwig de Pesquisa sobre o Câncer/BR / Universidade Federal de Minas Gerais/BR

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Full text: Available Index: LILACS (Americas) Main subject: Schistosoma mansoni / Biomphalaria / Polymorphism, Restriction Fragment Length / RNA, Ribosomal / Polymerase Chain Reaction / Electron Transport Complex IV Limits: Animals Country/Region as subject: South America / Argentina / Brazil / Uruguay Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2002 Type: Article / Congress and conference Affiliation country: Brazil Institution/Affiliation country: Fiocruz/BR / Instituto Ludwig de Pesquisa sobre o Câncer/BR / Universidade Federal de Minas Gerais/BR