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A tobacco cDNA reveals two different transcription patterns in vegetative and reproductive organs
Silva, I. da; Angelo, P. C. S; Molfetta, J. B; Ferraz, M. T; Silva, L. L. P. da; Goldman, G. H; Goldman, M. H. S.
  • Silva, I. da; Universidade de Säo Paulo. Faculdade de Filosofia, Ciências e Letras de Ribeiräo Preto. Departamento de Biologia. Ribeiräo Preto. BR
  • Angelo, P. C. S; Universidade de Säo Paulo. Faculdade de Filosofia, Ciências e Letras de Ribeiräo Preto. Departamento de Biologia. Ribeiräo Preto. BR
  • Molfetta, J. B; Universidade de Säo Paulo. Faculdade de Filosofia, Ciências e Letras de Ribeiräo Preto. Departamento de Biologia. Ribeiräo Preto. BR
  • Ferraz, M. T; Universidade de Säo Paulo. Faculdade de Filosofia, Ciências e Letras de Ribeiräo Preto. Departamento de Biologia. Ribeiräo Preto. BR
  • Silva, L. L. P. da; Universidade de Säo Paulo. Faculdade de Filosofia, Ciências e Letras de Ribeiräo Preto. Departamento de Biologia. Ribeiräo Preto. BR
  • Goldman, G. H; Universidade de Säo Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Ciências Farmacêuticas. Ribeiräo Preto. BR
  • Goldman, M. H. S; Universidade de Säo Paulo. Faculdade de Filosofia, Ciências e Letras de Ribeiräo Preto. Departamento de Biologia. Ribeiräo Preto. BR
Braz. j. med. biol. res ; 35(8): 861-868, Aug. 2002. ilus
Article in English | LILACS | ID: lil-325533
RESUMO
In order to identify genes expressed in the pistil that may have a role in the reproduction process, we have established an expressed sequence tags project to randomly sequence clones from a Nicotiana tabacum stigma/style cDNA library. A cDNA clone (MTL-8) showing high sequence similarity to genes encoding glycine-rich RNA-binding proteins was chosen for further characterization. Based on the extensive identity of MTL-8 to the RGP-1a sequence of N. sylvestris, a primer was defined to extend the 5' sequence of MTL-8 by RT-PCR from stigma/style RNAs. The amplification product was sequenced and it was confirmed that MTL-8 corresponds to an mRNA encoding a glycine-rich RNA-binding protein. Two transcripts of different sizes and expression patterns were identified when the MTL-8 cDNA insert was used as a probe in RNA blots. The largest is 1,100 nucleotides (nt) long and markedly predominant in ovaries. The smaller transcript, with 600 nt, is ubiquitous to the vegetative and reproductive organs analyzed (roots, stems, leaves, sepals, petals, stamens, stigmas/styles and ovaries). Plants submitted to stress (wounding, virus infection and ethylene treatment) presented an increased level of the 600-nt transcript in leaves, especially after tobacco necrosis virus infection. In contrast, the level of the 1,100-nt transcript seems to be unaffected by the stress conditions tested. Results of Southern blot experiments have suggested that MTL-8 is present in one or two copies in the tobacco genome. Our results suggest that the shorter transcript is related to stress while the larger one is a flower predominant and nonstress-inducible messenger
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Full text: Available Index: LILACS (Americas) Main subject: Plant Proteins / Reproduction / Tobacco / RNA-Binding Proteins / Flowers Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2002 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de Säo Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Plant Proteins / Reproduction / Tobacco / RNA-Binding Proteins / Flowers Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2002 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de Säo Paulo/BR