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Evaluation of the direct agglutination test and the rK39 dipstick test for the sero-diagnosis of visceral leishmaniasis
Schallig, Henk D. F. H; Canto-Cavalheiro, Marilene; Silva, Eduardo S da.
  • Schallig, Henk D. F. H; Biomedical Research. Koninklijk Instituut voor de Tropen. Amsterdam. NL
  • Canto-Cavalheiro, Marilene; Fiocruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro. BR
  • Silva, Eduardo S da; Fiocruz. Centro de Pesquisas René Rachou. Laboratório de Leishmanioses. Belo Horizonte. BR
Mem. Inst. Oswaldo Cruz ; 97(7): 1015-1018, Oct. 2002. tab
Article in English | LILACS | ID: lil-325912
RESUMO
The direct agglutination test (DAT) based on a freeze-dried antigen and the rK39 dipstick test were evaluated for the sero-diagnosis of visceral leishmaniasis (VL). The sensitivity and specificity of both tests were determined using sera from confirmed VL patients (n = 21), healthy controls (n = 19) and from patients with other confirmed infectious diseases (n = 42). The DAT had a sensitivity and a specificity of 100 percent. The rK39 had a sensitivity of 85.7 percent and a specificity of 82 percent. Both tests were also used to screen blood samples of confirmed VL patients (n = 15) and serum samples of VL suspects (n = 61). The DAT found all blood samples of confirmed VL patients positive and tested 98.4 percent of the serum samples of the VL suspects positive. In contrast, rK39 detected in 9/15 blood samples (60 percent) antibodies against Leishmania chagasi and found 85.3 percent of the serum samples of the suspected patients positive. Although the rK39 dipstick is more rapid and user friendlier than the DAT, the latter has a superior sensitivity and specificity. Furthermore, the reagents used for DAT do not require cold storage, whereas the buffer of the rK39 must be stored at 4ºC. Therefore, the DAT is the most suitable test for the sero-diagnosis of VL under field conditions
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Leishmania donovani / Antibodies, Protozoan / Protozoan Proteins / Leishmaniasis, Visceral Type of study: Diagnostic study / Evaluation studies / Observational study Limits: Animals / Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2002 Type: Article Affiliation country: Brazil / Netherlands Institution/Affiliation country: Biomedical Research/NL / Fiocruz/BR

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Full text: Available Index: LILACS (Americas) Main subject: Leishmania donovani / Antibodies, Protozoan / Protozoan Proteins / Leishmaniasis, Visceral Type of study: Diagnostic study / Evaluation studies / Observational study Limits: Animals / Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2002 Type: Article Affiliation country: Brazil / Netherlands Institution/Affiliation country: Biomedical Research/NL / Fiocruz/BR