Detection of antibodies to the 97 kDa component of Toxoplasma gondii in samples of human serum
Mem. Inst. Oswaldo Cruz
;
97(7): 1009-1013, Oct. 2002. ilus, tab
Article
in English
| LILACS
| ID: lil-325915
RESUMO
This study was carried out to investigate the immune response against 97 kDa (p97) molecular marker of Toxoplasma gondii that has been characterized as a cytosolic protein and a component of the excreted-secreted antigens from this parasite. A total of 60 serum samples from patients were analyzed by enzime-linked immunosorbent assay and Western blot for toxoplasmosis. These samples were organized in three groups, based on clinical symptoms and results of serological tests. Group I 20 samples reactive to IgG and IgM (acute phase); group II 20 non-reactive samples (control group); and group III 20 samples reactive only to IgG (chronic phase). Western blot was performed with total antigenic extracts or with excreted and secreted antigen from T. gondii to identify the fraction correspondent to p97. It was observed that this cytosolic component from T. gondii stimulates the immunologic system to produce both IgM and IgG antibodies in the beginning of the acute infection and IgG throughout the chronic stage of the asymptomatic toxoplasmosis
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Toxoplasma
/
Antibodies, Protozoan
/
Protozoan Proteins
/
Toxoplasmosis
Type of study:
Diagnostic study
/
Observational study
Limits:
Animals
/
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2002
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Uberlândia/BR
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