Complete nucleotide sequence analysis of a Brazilian dengue virus type 2 strain
Mem. Inst. Oswaldo Cruz
;
97(7): 991-995, Oct. 2002. ilus, tab
Article
in English
| LILACS
| ID: lil-325922
RESUMO
In the last decade, dengue fever (DF) in Brazil has been recognized as an important public health problem, and an increasing number of dengue haemorrhagic fever (DHF) cases have been reported since the introduction of dengue virus type 2 (DEN-2) into the country in 1990. In order to analyze the complete genome sequence of a DEN-2 Brazilian strain (BR64022/98), we designed primers to amplify contiguous segments of approximately 500 base pairs across the entire sequence of the viral genome. Twenty fragments amplified by reverse transcriptase-PCR were cloned, and the complete nucleotide and the deduced amino acid sequences were determined. This constitutes the first complete genetic characterization of a DEN-2 strain from Brazil. All amino acid changes differentiating strains related to the Asian/American-Asian genotype were observed in BR64022/98, indicating the Asiatic origin of the strain
Full text:
Available
Index:
LILACS (Americas)
Main subject:
RNA, Viral
/
Base Sequence
/
Amino Acid Sequence
/
Genome, Viral
/
Dengue Virus
Limits:
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2002
Type:
Article
/
Project document
Affiliation country:
Brazil
/
United States
Institution/Affiliation country:
Fiocruz/BR
/
University of California/US
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