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Effect of perimuscular injection of Bothrops jararacussu venom on plasma creatine kinase levels in mice: influence of dose and volume
Calil-Elias, S; Thattassery, E; Martinez, A. M. B; Melo, P. A.
  • Calil-Elias, S; Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Farmacologia Basica e Clinica. Rio de Janeiro. BR
  • Thattassery, E; Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Farmacologia Basica e Clinica. Rio de Janeiro. BR
  • Martinez, A. M. B; Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Histologia e Embriologia. Rio de Janeiro. BR
  • Melo, P. A; Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Farmacologia Basica e Clinica. Rio de Janeiro. BR
Braz. j. med. biol. res ; 35(10): 1233-1235, Oct. 2002. ilus, graf
Article in English | LILACS | ID: lil-326231
ABSTRACT
The effect of dose and volume of a perimuscular injection of Bothrops jararacussu venom on myonecrosis of skeletal muscle was studied in mice. An increase of the venom dose (0.25 to 2.0 æg/g) at a given volume (50 æl) resulted in an increase in plasma creatine kinase (CK) levels 2 h after injection. Plasma CK activity increased from the basal level of 129.27 ± 11.83 (N = 20) to 2392.80 ± 709.43 IU/l (N = 4) for the 1.0 æg/g dose. Histological analysis of extensor digitorum longus muscle 4 h after injection showed lesion of peripheral muscle fibers, disorganization of the bundles or the complete degeneration of muscle fibers. These lesions were more extensive when higher doses were injected. Furthermore, an increase in volume (12.5 to 100 æl) by dilution of a given dose (0.5 æg/g) also increased plasma CK levels from 482.31 ± 122.79 to 919.07 ± 133.33 IU/l (N = 4), respectively. These results indicate that care should be taken to standardize volumes and sites of venom injections
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Bothrops / Muscle, Skeletal / Creatine Kinase / Crotalid Venoms Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2002 Type: Article / Congress and conference Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Rio de Janeiro/BR

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Full text: Available Index: LILACS (Americas) Main subject: Bothrops / Muscle, Skeletal / Creatine Kinase / Crotalid Venoms Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2002 Type: Article / Congress and conference Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Rio de Janeiro/BR