Detection of hilA gene sequences in serovars of Salmonella enterica sufigbspecies enterica
Mem. Inst. Oswaldo Cruz
;
97(8): 1153-1156, Dec. 15, 2002. ilus
Article
in English
| LILACS
| ID: lil-326326
ABSTRACT
hilA gene promoter, component of the Salmonella Pathogenicity Island 1, has been found in Salmonella serovar Typhimurium, being important for the regulation of type III secretion apparatus genes. We detected hilA gene sequences in Salmonella serovars Typhi, Enteritidis, Choleraesuis, Paratyphi A and B, and Pullorum, by polymerase chain reaction (PCR) and hybridization techniques. The primers to carry out PCR were designed according to hilA sequence. A low stringency hybridization with the probe pVV441 (hilA open-reading-frame plasmid) was carried out. To find hilA gene sequences in other Salmonella sp. suggest that these serovars could have similar sequences of this kind of virulence genes
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Bacterial Proteins
/
Trans-Activators
/
Salmonella enterica
Type of study:
Diagnostic study
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2002
Type:
Article
/
Project document
Affiliation country:
Colombia
Institution/Affiliation country:
Instituto Colombiano de Medicina Tropical/CO
/
Universidad de Antioquia/CO
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