Mtp-40 and alpha antigen gene fragment amplification for the detection of Mycobacterium tuberculosis in Colombian clinical specimens
Mem. Inst. Oswaldo Cruz
;
97(8): 1157-1163, Dec. 15, 2002. ilus, tab
Article
in English
| LILACS
| ID: lil-326328
RESUMO
In this study, the use of Mtp-40 and alpha antigen polymerase chain reaction (PCR) amplification fragments for the precise tuberculosis (TB) diagnosis was evaluated. One hundred and ninety two different samples were obtained from 113 patients with suspected TB. Mtp-40 and alpha antigen protein genes were amplified by the PCR technique and compared to both the "gold standard" (culture) test, as well as the clinical parameters (including a clinical record and X-ray film exam in 113 patients). Thirty-eight of the 113 patients had a presumptive clinical diagnosis of TB; 74 percent being detected by PCR technique, 58 percent by culture and 44 percent by direct microscopic visualization. Weconclude that it is possible to use PCR as a suitable technique for the detection of any mycobacteria by means of the alpha antigen product, or the specific infection of Mycobacterium tuberculosis by means of the mtp-40 gene. This might be a good supporting tool in difficult clinical TB diagnosis and pauci-bacillary cases
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Bacterial Proteins
/
Tuberculosis
/
Mycobacterium tuberculosis
/
Antigens, Bacterial
Type of study:
Diagnostic study
/
Evaluation studies
Limits:
Humans
Country/Region as subject:
South America
/
Colombia
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2002
Type:
Article
Affiliation country:
Colombia
Institution/Affiliation country:
Universidad Nacional de Colombia/CO
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