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Conventional and molecular typing of Salmonella typhi strains from Brazil
Quintaes, Bianca R; Leal, Nilma C; Reis, Eliane M. F; Fonseca, Érica L; Hofer, Ernesto.
  • Quintaes, Bianca R; Fiocruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Rio de Janeiro. BR
  • Leal, Nilma C; Fiocruz. Centro de Pesquisas Aggeu Magalhões. Laboratório de Microbiologia. Recife. BR
  • Reis, Eliane M. F; Fiocruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Rio de Janeiro. BR
  • Fonseca, Érica L; Fiocruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Rio de Janeiro. BR
  • Hofer, Ernesto; Fiocruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Rio de Janeiro. BR
Rev. Inst. Med. Trop. Säo Paulo ; 44(6): 315-319, Nov.-Dec. 2002. ilus, tab
Article in English | LILACS | ID: lil-326349
RESUMO
Phenotypic and genotypic characteristics of Salmonella Typhi were studied in 30 strains, isolated in different years, from some areas in Brazil. Conventional typing methods were performed by biochemical tests, Vi phage-typing scheme, and antimicrobial susceptibility test. Molecular typing methods were performed by analysis of plasmid DNA and by random amplified polymorphic DNA (RAPD-PCR). For the latter, an optimization step was performed to ensure the reproducibility of the process in genetic characterization of S. Typhi. The predominance of 76.7 percent of biotype I (xylose +, arabinose -) was noticed in all studied areas. Three phage types were recognized, with prominence for the phage types A (73.3 percent) and I+IV (23.3 percent). All the strains were susceptible to the drugs used. However, 36.7 percent of the strains contained plasmids, with predominance of the 105 Kb plasmid. RAPD was capable of grouping the strains in 8 genotypic patterns using primer 784, in 6, using primer 787 and in 7, using primer 797. Conventional phenotypic typing methods, as well as the DNA plasmid analysis, presented nonsignificant discriminatory power; however, RAPD-PCR analysis showed discriminatory power, reproducibility, easy interpretation and performance, being considered as a promising alternative typing method for S. Typhi
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Salmonella typhi / Bacterial Typing Techniques Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 2002 Type: Article Affiliation country: Brazil Institution/Affiliation country: Fiocruz/BR

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Full text: Available Index: LILACS (Americas) Main subject: Salmonella typhi / Bacterial Typing Techniques Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 2002 Type: Article Affiliation country: Brazil Institution/Affiliation country: Fiocruz/BR