Design and applications of modified oligonucleotides
Braz. j. med. biol. res
;
36(2): 143-151, Feb. 2003. ilus
Article
in English
| LILACS
| ID: lil-326425
RESUMO
Oligonucleotides have a wide range of applications in fields such as biotechnology, molecular biology, diagnosis and therapy. However, the spectrum of uses can be broadened by introducing chemical modifications into their structures. The most prolific field in the search for new oligonucleotide analogs is the antisense strategy, where chemical modifications confer appropriate characteristics such as hybridization, resistance to nucleases, cellular uptake, selectivity and, basically, good pharmacokinetic and pharmacodynamic properties. Combinatorial technology is another research area where oligonucleotides and their analogs are extensively employed. Aptamers, new catalytic ribozymes and deoxyribozymes are RNA or DNA molecules individualized from a randomly synthesized library on the basis of a particular property. They are identified by repeated cycles of selection and amplification, using PCR technologies. Modified nucleotides can be introduced either during the amplification procedure or after selection
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Oligonucleotides
/
Drug Design
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2003
Type:
Article
/
Congress and conference
Affiliation country:
Argentina
Institution/Affiliation country:
Universidad de Buenos Aires/AR
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