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Absence of peripheral blood mononuclear cells priming in hemodialysis patients
Santos, B. C; Starobinas, N; Barbuto, J. A. M; Russo, M; Schor, N.
  • Santos, B. C; Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Medicina. Disciplina de Nefrologia. São Paulo. BR
  • Starobinas, N; Instituto Butantan. Laboratório de Imunogenética. São Paulo. BR
  • Barbuto, J. A. M; Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia. São Paulo. BR
  • Russo, M; Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia. São Paulo. BR
  • Schor, N; Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Medicina. Disciplina de Nefrologia. São Paulo. BR
Braz. j. med. biol. res ; 36(2): 219-225, Feb. 2003. graf
Article in English | LILACS, SES-SP | ID: lil-326427
RESUMO
As a consequence of the proinflammatory environment occurring in dialytic patients, cytokine overproduction has been implicated in hemodialysis co-morbidity. However, there are discrepancies among the various studies that have analyzed TNF-alpha synthesis and the presence of peripheral blood mononuclear cell (PBMC) priming in this clinical setting. We measured bioactive cytokine by the L929 cell bioassay, and evaluated PBMC TNF-alpha production by 32 hemodialysis patients (HP) and 51 controls. No difference in TNF-alpha secretion was observed between controls and HP (859 ± 141 vs 697 ± 130 U/10(6) cells). Lipopolysaccharide (5 æg/ml) did not induce any further TNF-alpha release, showing no PBMC priming. Paraformaldehyde-fixed HP PBMC were not cytotoxic to L929 cells, suggesting the absence of membrane-anchored TNF-alpha. Cycloheximide inhibited PBMC cytotoxicity in HP and controls, indicating lack of a PBMC TNF-alpha pool, and dependence on de novo cytokine synthesis. Actinomycin D reduced TNF-alpha production in HP, but had no effect on controls. Therefore, our data imply that TNF-alpha production is an intrinsic activity of normal PBMC and is not altered in HP. Moreover, TNF-alpha is a product of de novo synthesis by PBMC and is not constitutively expressed on HP cell membranes. The effect of actinomycin D suggests a putative tighter control of TNF-alpha mRNA turnover in HP. This increased dependence on TNF-alpha RNA transcription in HP may reflect an adaptive response to hemodialysis stimuli
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Full text: Available Index: LILACS (Americas) Main subject: Leukocytes, Mononuclear / Cytokines / Renal Dialysis / Tumor Necrosis Factor-alpha Type of study: Observational study Limits: Adult / Humans Language: English Journal: Braz. j. med. biol. res Year: 2003 Type: Article Institution/Affiliation country: Instituto Butantan/BR / Universidade Federal de São Paulo/BR / Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Leukocytes, Mononuclear / Cytokines / Renal Dialysis / Tumor Necrosis Factor-alpha Type of study: Observational study Limits: Adult / Humans Language: English Journal: Braz. j. med. biol. res Year: 2003 Type: Article Institution/Affiliation country: Instituto Butantan/BR / Universidade Federal de São Paulo/BR / Universidade de São Paulo/BR