Primary culture of hepatic cells from Metynnis roosevelti (Pisces, Teleostei, Characidae)
Braz. j. vet. res. anim. sci
;
37(5): 366-371, 2000. ilus
Article
in English
| LILACS
| ID: lil-327432
RESUMO
In order to achieve monolayers and adhesion in cell cultures of hepatocytes from the subtropical fish Metynnis roosevelti (Pisces, Teleostei, Characidae) three different methodologies were used enzyme cell dissociation by means of a system containing trypsin and versene 0.25 percent; by means of bovine fetal serum and by the use of blood serum obtained from adult specimens of M. roosevelti. The cell medium used has been a modified F10-199 by the addition of 2 mM L-glutamine, 10 µg/mL insulin, 50 µg/mL fibronectin; and antibiotics. The best results in terms of cell dissociation as well as in terms of adhesion and confluence of the cells monolayer has been achieved by means of the enzymatic cell dissociation
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Cells
/
Cell Culture Techniques
/
Fishes
/
Liver
Language:
English
Journal:
Braz. j. vet. res. anim. sci
Journal subject:
Veterinary Medicine
Year:
2000
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Pontifícia Universidade Católica do Paraná/BR
/
Universidade Federal do Paraná/BR
Similar
MEDLINE
...
LILACS
LIS