Molecular characterization of Dengue viruses type 1 and 2 isolated from a concurrent human infection
Rev. Inst. Med. Trop. Säo Paulo
;
45(1): 11-16, Jan.- Feb. 2003. ilus
Article
in English
| LILACS
| ID: lil-330506
ABSTRACT
In 2001, an autochthonous case of dual viremia, resulting from naturally acquired dengue virus DEN-1 and DEN-2 infections was detected during the dengue outbreak that occurred in Barretos, a city with about 105,000 inhabitants in the North region of São Paulo State. Serotype identification was based on virus isolation to C6/36 mosquito cells culture and immunofluorescence assays using type-specific monoclonal antibodies. The double infection was also confirmed by reverse transcriptase polymerase chain reaction (RT-PCR). Comparative analysis of the 240-nucleotide sequences of E/NS1 gene junction region between the genome of DEN-1 and DEN-2 isolates of the corresponding reference Nauru and PR 159S1 strains, respectively, showed some nucleotide differences, mainly silent mutations in the third codon position. Results of maximum likelihood phylogenetic analysis of E/NS1 gene sequences indicated that both genotypes of DEN-1 and DEN-2 viruses recovered from double infection in Barretos belonged to genotypes I and III, respectively
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Dengue
/
Dengue Virus
Type of study:
Prognostic study
Limits:
Adolescent
/
Humans
/
Male
Language:
English
Journal:
Rev. Inst. Med. Trop. Säo Paulo
Journal subject:
Tropical Medicine
Year:
2003
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto Adolfo Lutz/BR
/
Universidade de São Paulo/BR
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