Staining of intestinal protozoa with heidenhain's iron Hematoxylin
Rev. Inst. Med. Trop. Säo Paulo
;
45(1): 43-44, Jan.- Feb. 2003.
Article
in English
| LILACS
| ID: lil-330512
ABSTRACT
Due to its unique properties, iron hematoxylin has been traditionally recommended for staining intestinal protozoa. This process can be simplified by reducing the number of steps and periods of permanence of the slides in some of the liquids used, without detriment to the quality of the results. Thus iron hematoxylin becomes adequate for routine use. Hematoxylin is a natural dye extracted from Haematoxylon campechianum, of the family Leguminosae. It must first be 'ripened', i.e. oxidized to hematein, which reacts with ferric ammonium sulphate to produce the ferric lake (iron hematoxylin), a basic dye. Iron hematoxylin most frequently stains regressively, i.e. the slides are first overstained and then differentiated
Full text:
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Index:
LILACS (Americas)
Main subject:
Staining and Labeling
/
Coloring Agents
/
Eukaryota
/
Hematoxylin
Limits:
Animals
Language:
English
Journal:
Rev. Inst. Med. Trop. Säo Paulo
Journal subject:
Tropical Medicine
Year:
2003
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade de São Paulo/BR
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