Microsporogénesis y microgametogénesis de onoto (Bixa orellana L. ) / Microsporogenesis and microgametogenesis of annatto (Bixa orellana L. )
Acta cient. venez
;
53(3): 171-175, 2002. ilus
Article
in Spanish
| LILACS
| ID: lil-331334
ABSTRACT
A series of buds of increasing maturity were individually sampled in order to examine cytological events of annatto (Bixa orellana L.), genotype Portuguesa. They were fixed in Carnoy II at 12:30 am, time of the highest rate of meiotic division. Three stain solutions were attempted. In the microspores mother cells, the use of acetic orcein 1% resulted in a good nucleus coloration and sharpness. In contrast, a well chromosome resolution was achieved with the application of propionic carmin 2%. The pollen grain mother cells (n = 8 chromosomes) at metaphase I were found in floral buds of 0.5 to 0.6 cm long; tetrad stage in buds of 0.6 to 0.7 cm long, uninucleate stage of microspores in buds of 0.7 to 0.8 cm long and the binucleate stage (pollen) in buds longer than 0.8 cm. Microphotographies showing the sequence of meiotic division (microsporogenesis) and subsequent mitosis to originate pollen grains were included
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Pollen
/
Cell Division
/
Chromosomes
/
Bixaceae
Language:
Spanish
Journal:
Acta cient. venez
Journal subject:
Science
Year:
2002
Type:
Article
Affiliation country:
Venezuela
Institution/Affiliation country:
Universidad Central de Venezuela/VE
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