Prenatal toxoplasmosis diagnosis from amniotic fluid by PCR
Rev. Soc. Bras. Med. Trop
;
35(1): 1-6, jan.-fev. 2002.
Article
in English
| LILACS
| ID: lil-331785
RESUMO
Toxoplasmosis is one of the most common infections all over the world. Most cases are asymptomatic, except in immunosuppressed individuals and fetuses, which can be seriously damaged. Prenatal diagnosis should be made as soon as possible since treatment of the mother can minimize fetal sequelae. Our aim in this study was to test the polymerase chain reaction technique (PCR) in 86 samples of amniotic fluid from women who seroconverted during pregnancy. DNA was amplified using external primers and, in a second step, internal primers, in a nested PCR system. Samples were also inoculated into mice and the newborn were evaluated by T. gondii serology, skull x-ray, transfontanel ultrasound, fundoscopic examination, lumbar puncture and clinical examination. PCR was positive in seven cases and negative in 79. Among PCR-positive cases, two were negative by inoculation into mice and by clinical evaluation; among PCR-negative ones, three had clinical evidence of toxoplasmosis and one was positive after inoculation into mice. PCR showed values of sensitivity = 62.5 and specificity = 97.4; the values of inoculation into mice where 42.9 and 100, respectively. Although PCR should not be used alone for prenatal diagnosis of congenital toxoplasmosis, it is a promising method and deserves more studies to improve its efficacy.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Prenatal Diagnosis
/
Toxoplasmosis
/
Fetal Diseases
Type of study:
Diagnostic study
Limits:
Adult
/
Female
/
Humans
/
Male
/
Infant, Newborn
/
Pregnancy
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2002
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Minas Gerais/BR
/
universidade Federal de Minas Gerais/BR
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