Characterization of enteroaggregative Escherichia coli strains isolated from children with acute diarrhea, in Mendoza, Argentina
Rev. argent. microbiol
;
34(3): 167-170, jul.-sept. 2002.
Article
in English
| LILACS
| ID: lil-331787
RESUMO
Enteroaggregative Escherichia coli (EAEC) is an increasingly recognized cause of diarrhea in children in developing and developed countries. EAEC is recognized by a characteristic aggregative pattern of adherence to human epithelial (HEp-2) cells cultured in vitro. This is the gold standard assay. The aggregative phenotype is associated with the presence of a 65 MDa plasmid (pAA) that also encodes several other putative virulence factors, such as the aggregative adherence fimbria I (AAF/I) and the enteroaggregative heat-stable enterotoxin (EAST1). The objective of this work was to evaluate the application of PCR (polymerase chain reaction) to identify EAEC strains in cases of acute diarrhea. A total of 87 E. coli strains, isolated from patients under 2 years of age with acute diarrhea in Mendoza, Argentina, were characterized by the reference method (HEp-2 assay), and by AAF/I- and EAST1-PCR. PCR sensitivity and specificity in comparison with the cell culture assay showed 94.4 sensitivity and 78.26 specificity. EAST1- and AAF/I-PCR could be recommended as a screening test, applicable to epidemiologic studies.
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Index:
LILACS (Americas)
Main subject:
Bacterial Adhesion
/
Diarrhea, Infantile
/
Escherichia coli
/
Escherichia coli Infections
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Limits:
Humans
/
Infant
Country/Region as subject:
South America
/
Argentina
Language:
English
Journal:
Rev. argent. microbiol
Journal subject:
Microbiology
Year:
2002
Type:
Article
Affiliation country:
Argentina
Institution/Affiliation country:
Hospital Materno Infantil Dr. Humberto Notti/AR
/
Universidad Nacional de Cuyo/AR
/
Universidad de San Luis/AR
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