Detección y genotipificación del papillomavirus humano (HPV) por PCR-LIS-SSCP / Detection and genotyping of human papillomavirus (HPV) using PCR-LIS-SSCP
Rev. argent. microbiol
;
33(1): 22-27, ene.-mar. 2001.
Article
in Spanish
| LILACS
| ID: lil-332505
ABSTRACT
This study describes a fast and simple method for human papillomavirus (HPV) typing based on the polymerase chain reaction (PCR) amplification of a portion of the viral genome and single strand conformation polymorphism using low ionic strength solutions (LIS-SSCP). PCR products were obtained using My09/My11 and Gp5/Gp6 primers in a nested reaction. The band patterns corresponded to the plasmid HPV clones from HPV-6, -11, -16, -18, -31, -33 and -34. The SSCP minigels were stained with SYBR-Green II. In order to determine diagnostic applicability, 100 cervical samples were studied comprising liquid cytology and paraffin embedded biopsies from patients showing squamous intraepithelial lesions (SILs). The SSCP patterns obtained from the clinical samples and the HPV clones were similar when the same type was present. Therefore, the methodology proved to be efficient and with high reproducibility for the detection and typing of HPV in clinical samples.
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Index:
LILACS (Americas)
Main subject:
Papillomaviridae
/
Tumor Virus Infections
/
Cervix Uteri
/
Polymerase Chain Reaction
/
Genome, Viral
/
Polymorphism, Single-Stranded Conformational
/
Papillomavirus Infections
Type of study:
Diagnostic study
Limits:
Female
/
Humans
Language:
Spanish
Journal:
Rev. argent. microbiol
Journal subject:
Microbiology
Year:
2001
Type:
Article
Affiliation country:
Argentina
Institution/Affiliation country:
Universidad Nacional de La Plata/AR
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