Expresión y caracterización de dos proteínas estructurales del virus de la diarrea viral bovina (VDVB) / Expression and characterization of 2 bovine viral diarrhea virus structural proteins (]BVDV)
Rev. argent. microbiol
;
33(1): 15-21, ene.-mar. 2001.
Article
in Spanish
| LILACS
| ID: lil-332506
RESUMO
The BVDV glycoproteins gp48 and gp53 were expressed in the baculovirus eukaryotic system. Both recombinant proteins were recognized in western blot analysis by monoclonal antibodies and polyclonal serum. Immunofluorescent test demonstrated that gp53 was localized on the cell surface whereas gp48 was in the cytoplasm. The expressed proteins were extracted by non-denaturing detergent treatment. Rabbit antiserum raised against gp53 recombinant protein efficiently neutralized the virus. These results demonstrate that the recombinant proteins have immunological properties similar to those of the native viral protein and that they can be useful as diagnostic reagents.
Search on Google
Index:
LILACS (Americas)
Main subject:
Viral Envelope Proteins
/
Diarrhea Viruses, Bovine Viral
Limits:
Animals
Language:
Spanish
Journal:
Rev. argent. microbiol
Journal subject:
Microbiology
Year:
2001
Type:
Article
Affiliation country:
Argentina
Institution/Affiliation country:
CICVyA/AR
Similar
MEDLINE
...
LILACS
LIS