In vitro micropropagation of Lawsonia inermis (Lythraceae)
Rev. biol. trop
;
49(3/4): 957-963, Sep.-Dec. 2001.
Article
in English
| LILACS
| ID: lil-333091
ABSTRACT
A successful protocol was developed for mass propagation of Lawsonia inermis Linn., an important medicinal plant. Multiple shoots were induced in apical and axillary meristems derived from mature explants of L. inermis on Murashige and Skoog (1962) medium supplemented with 0.25 mg/l 6-benzylaminopurine (BA), 0.25 mg/l Kinetin (Kn), 0.5 mg/l ascorbic acid and 3 (w/v) sucrose. The rate of multiplication was higher when the cultures were incubated under continuous light rather than the 14 hr photoperiod. Rooting was readily achieved upon transferring the microshoots onto MS basal semi-solid medium supplemented with 0.25 mg/l indole-3-butyric acid (IBA) after ten days of culture. Micropropagated plantlets were acclimatized and successfully grown in soil.
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Index:
LILACS (Americas)
Main subject:
Plant Shoots
/
Lythraceae
Language:
English
Journal:
Rev. biol. trop
Journal subject:
Biology
/
Tropical Medicine
Year:
2001
Type:
Article
Affiliation country:
India
Institution/Affiliation country:
Plant Physiology and Biochemistry Laboratory/IN
/
Plant Tissue Culture Laboratory/IN
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