Obtención de un conjugado látex inmunoglobulina para el diagnóstico de Gardnerella vaginalis / The production of a latex immunoglobulin conjugate for the diagnosis of Gardnerella vaginalis
Rev. cuba. med. trop
;
52(2): 101-105, May-Aug. 2000.
Article
in Spanish
| LILACS
| ID: lil-333489
ABSTRACT
To obtain an anti-Gadnerella vaginalis latex globulin, specific immunosera to reference strain (ATCC 14018) and to a clinical isolation strain pool were produced. These immunosera were characterized using PAGE-SDS and immunoblotting where a close antigenic relation between the clinical isolation strains and the reference strain was observed. The latex globulin conjugates obtained from these 2 immunosera were evaluated in vitro with a resulting level of detection of 10 ufc/mL of Gardnerella vaginalis. These two conjugates were also evaluated in clinical samples and compared with the 6 vaginalis culture and the criteria considered for diagnosing bacterial vaginosis. The anti-strain pool (II) latex globulin conjugate turned out to be more sensitive and specific than anti-G. vaginalis ATCC strain (I) latex globulin.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Immunoglobulins
/
Gardnerella vaginalis
/
Vaginosis, Bacterial
/
Latex
Type of study:
Diagnostic study
Limits:
Animals
/
Female
/
Humans
Language:
Spanish
Journal:
Rev. cuba. med. trop
Journal subject:
Tropical Medicine
Year:
2000
Type:
Article
Affiliation country:
Cuba
Institution/Affiliation country:
Centro Nacional de Sanidad Agropecuaria/CU
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