Detección rápida de Enterovirus mediante un método directo de reacción en cadena de la polimerasa / Rapid detection of Enterovirus by a direct method of polymerase chain reaction
Rev. cuba. med. trop
;
52(1): 15-20, Jan.-Apr. 2000.
Article
in Spanish
| LILACS
| ID: lil-333503
ABSTRACT
For the detection of Enterovirus, we devised a direct economical method of polymerase chain reaction which does not require a previous extraction of ribonucleic acid and uses infected cell culture supernatants. The system was developed on the basis of universal primers of Enterovirus and specific primers of vaccinal strain Sabin 1. The achieved results proved that the non-existence of methods of RNA extraction and purification prior to the reaction does not affect the susceptibility and specificity of the system, in the rapid detection of Enterovirus genomes and identification of vaccinal strains of poliovirus.
Full text:
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Index:
LILACS (Americas)
Main subject:
Polymerase Chain Reaction
/
Enterovirus
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
Spanish
Journal:
Rev. cuba. med. trop
Journal subject:
Tropical Medicine
Year:
2000
Type:
Article
Affiliation country:
Cuba
Institution/Affiliation country:
Instituto de Medicina Tropical Pedro Kouri/CU
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