Producción y purificación parcial de la hemolisina principal (Neumolisina) de Streptococcus pneumoniae / Production and partial purification of the main hemolysin (pneumolysin) of Streptococcus pneumoniae

ABSTRACT

The capacity of 36 Cuban strains of Streptococcus pneumoniae to produce pneumolysin was studied, and 94.4 of them were determined to be producers of that enzyme. One of the best producers was cultured at a great scale and the pneumolysin found in the supernatan was partially purified through an ion-exchange chromatography in mono-Q column. This method made it possible to recover the enzyme whose purity level increased by 4.39 with 100 output.