Diagnóstico serológico de la leptospirosis humana mediante 3 variantes de la técnica de hemaglutinación pasiva / Serologic diagnosis of human leptospirosis with 3 variants of the passive hemagglutination technique

ABSTRACT

A serological study was conducted to evaluate 3 variants of the positive hemagglutination technique (HA) with the use of formalized erythrocytes of fresh lamb (HC) and of formalized Type O, Rh-negative human red blood cells (HH). No significant statistical difference were found between the results of HA and HC and between HC and HH. Unspecific reaction was only observed in 2 of the 200 sera studied by HH and HC. Heterophil antibodies were just observed in 2.5 of the sera studied by HH, and in 100 of the sera studied by HC. The qualitative parameters were determined for the variants used, and this way the repetition and reproducibility of the most useful variant was evaluated.