Enterobius vermicularis: ancient DNA from north and south American human coprolites
Mem. Inst. Oswaldo Cruz
;
98(supl.1): 67-69, Jan. 15, 2003. ilus, tab
Article
in English
| LILACS
| ID: lil-333819
ABSTRACT
A molecular paleoparasitological diagnostic approach was developed for Enterobius vermicularis. Ancient DNA was extracted from 27 coprolites from archaeological sites in Chile and USA. Enzymatic amplification of human mtDNA sequences confirmed the human origin. We designed primers specific to the E. vermicularis 5S ribosomal RNA spacer region and they allowed reproducible polymerase chain reaction identification of ancient material. We suggested that the paleoparasitological microscopic identification could accompany molecular diagnosis, which also opens the possibility of sequence analysis to understand parasite-host evolution
Full text:
Available
Index:
LILACS (Americas)
Main subject:
DNA, Mitochondrial
/
RNA, Ribosomal, 5S
/
DNA, Helminth
/
Enterobius
/
Fossils
Limits:
Animals
/
Humans
Country/Region as subject:
North America
/
South America
/
Chile
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2003
Type:
Article
Affiliation country:
Brazil
/
United States
Institution/Affiliation country:
Fiocruz/BR
/
University of Nebraska/US
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