Preparation of sand fly (Diptera: Psychodidae: Phlebotominae) specimens for histological studies
Mem. Inst. Oswaldo Cruz
;
98(2): 287-290, Mar. 15, 2003. ilus, tab
Article
in English
| LILACS
| ID: lil-334270
ABSTRACT
Phlebotominae sand fly specimens were prepared for histological and physiological studies. Different fixatives were tested on sectioned and whole bodied adult females in order to obtain good fixation and provide satisfactory penetration of the embedding media. All fixed specimens were infiltrated (up to seven days under 5ºC) and embedded in hydroxyethyl metacrylate. Two-three æm sections were stained, mounted in Canada balsam and observed by light microscopy. Best results were achieved when whole bodied insects were double fixed in Bouin's and Carnoy's fluids (4 h/2 h) and stained in Hematoxilin/Eosin or fixed in calcium formaldehyde and stained in mercury bromophenol blue
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Index:
LILACS (Americas)
Main subject:
Psychodidae
/
Tissue Fixation
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2003
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Fiocruz/BR
/
Universidade Federal de Viçosa/BR
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