DNA synthesis and nucleolar organizer regions circadian rhythm in mouse regenerating liver hepatocytes
Biocell
;
24(2): 151-155, Aug. 2000.
Article
in English
| LILACS
| ID: lil-335901
ABSTRACT
DNA synthesis and Nucleolar Organizer Regions (NORs) were studied in C3HS inbred mice standardized for periodicity analysis. Immunohistochemical detection of Bromodeoxyuridine (BrdU) incorporated into DNA with a monoclonal antibody and silver staining of NORs (AgNORs) were assessed by means of a digital image analysis system in histological sections of regenerating liver. Tissue samples were obtained at different times after hepatectomy along a circadian span. The results showed a strong correlation of values between DNA synthesis (BrdU labelling index) and AgNOR numbers, with higher counts during the activity period of animals at 0000/38 and 0400/42 hours Time of Day/Hours Post-Hepatectomy (TD/HPH), being the differences with other time points highly significant. Our observations demonstrate the existence of a strong correlation of DNA synthesis measured by BrdU incorporation and AgNOR numbers with a defined circadian rhythm in mouse regenerating hepatocytes.
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Index:
LILACS (Americas)
Main subject:
Regeneration
/
DNA
/
Circadian Rhythm
/
Liver
/
Nucleolus Organizer Region
Limits:
Animals
Language:
English
Journal:
Biocell
Journal subject:
Clulas
Year:
2000
Type:
Article
Affiliation country:
Argentina
Institution/Affiliation country:
Universidad Nacional de La Plata/AR
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