Standardization of fixation, processing and staining methods for the central nervous system of vertebrates
Biocell
;
20(3): 265-272, Dec. 1996.
Article
in English
| LILACS
| ID: lil-335992
ABSTRACT
This paper reports the standardization of methods used for processing and embedding various vertebrate brains of different size in paraffin. Other technical details developed for avoiding frequent difficulties arising during laboratory routine are also reported. Some modifications of the Nissl and Klüver-Barrera staining methods are proposed. These modifications include 1) a Nissl stain solution with a rapid and efficient action with easier differentiation; 2) the use of a cheap microwave oven for the Klüver-Barrera stain. These procedures have the advantage of permitting Nissl and Klüver-Barrera staining of nervous tissue in about five and fifteen minutes respectively. The proposed procedures have been tested in brains obtained from fish, amphibians, reptiles and mammals of different body sizes. They are the result of our long experience in preparing slides for comparative studies. Serial sections of excellent quality were regularly obtained in all the specimens studied. These standardized methods, being simple and quick, are recommended for routine use in neurobiological laboratories.
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Index:
LILACS (Americas)
Main subject:
Staining and Labeling
/
Vertebrates
/
Central Nervous System
/
Tissue Fixation
/
Tissue Embedding
Limits:
Animals
Language:
English
Journal:
Biocell
Journal subject:
Clulas
Year:
1996
Type:
Article
Affiliation country:
Argentina
Institution/Affiliation country:
Universidad de Buenos Aires/AR
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