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Double replicas allow the obtention of longitudinally freeze-fractured Trypanosoma cruzi
Nascimento, A. E. do; Souza, W. de.
  • Nascimento, A. E. do; Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. BR
  • Souza, W. de; Universidade Estadual do Norte Fluminense. Centro de Biociências e Bioteconologia. Laboratório de Biologia Celular e Tecidual. Campos. BR
Biocell ; 20(3): 243-249, Dec. 1996.
Article in English | LILACS | ID: lil-335994
ABSTRACT
The double replica device was used to obtain freeze-fracture replicas of gently pressed cells, allowing the visualization of a large number of longitudinally fractured epimastigote and trypomastigote forms of Trypanosoma cruzi. This technique revealed large areas of the plasma membrane, the region of attachment of the flagellum to the cell body and the branched mitochondria.
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Index: LILACS (Americas) Main subject: Trypanosoma cruzi / Freeze Fracturing Limits: Animals Language: English Journal: Biocell Journal subject: C‚lulas Year: 1996 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual do Norte Fluminense/BR / Universidade Federal do Rio de Janeiro/BR

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Index: LILACS (Americas) Main subject: Trypanosoma cruzi / Freeze Fracturing Limits: Animals Language: English Journal: Biocell Journal subject: C‚lulas Year: 1996 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual do Norte Fluminense/BR / Universidade Federal do Rio de Janeiro/BR