Tubulin aggregates induced by Ni2+ present microtubular characteristics
Biocell
;
19(3): 183-188, Dec. 1995.
Article
in English
| LILACS
| ID: lil-336009
RESUMO
Rat brain tubulin in a proper buffered solution became insoluble in the presence of 10 mM NiCl2, and sedimented at centrifugal forces as low as 500 x g for 30 min. Both nickel-sedimented and microtubular tubulin conserved 65 of colchicine binding activity after 25 days of storage at -20 degrees C. However in brain cytosol, only 9 of the initial binding activity was conserved. The electrophoretic mobility of tubulin recovered from aggregates also remained unaltered. Therefore the aggregates formed with Ni2+ share important physicochemical properties with microtubules.
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Index:
LILACS (Americas)
Main subject:
Tubulin
/
Microtubules
/
Nickel
Limits:
Animals
Language:
English
Journal:
Biocell
Journal subject:
Clulas
Year:
1995
Type:
Article
Affiliation country:
Argentina
Institution/Affiliation country:
Universidad Nacional de Cuyo/AR
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