Pathogenesis-related proteins in Brazilian wheat genotypes: protein induction and partial gene sequencing
Ciênc. rural
;
33(3): 497-500, maio-jun. 2003. tab
Article
in English
| LILACS
| ID: lil-338909
ABSTRACT
Leaves from 14 Brazilian genotypes of Triticum aestivum L. were treated with salicylic acid to induce pathogenesis-related (PR) proteins. Inter and intracellular extracts were then obtained and investigated through polyacrilamide gel electrophoresis. Seven bands were observed. Material related to two of them (of 40 and 24 kDa) occurred in intracellular spaces only. DNA from these same genotypes was then amplified through PCR using primers developed from three sequences encoding PR proteins, and compared with previously described sequences. The fragments presented homologies to PR groups 1, 3 (chitinases), and 5 (thaumatin-like). The PR3-like sequence also showed a site characteristic of PRs induced by ethylene and a portion without homology with previous sequences. No variation among genotypes were observed, either for protein extracts or DNA sequences
Full text:
Available
Index:
LILACS (Americas)
Type of study:
Etiology study
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Ciênc. rural
Journal subject:
Science
/
Environmental Health
Year:
2003
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal do Rio Grande do Sul/BR
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