Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reaction
Mem. Inst. Oswaldo Cruz
;
98(3): 373-376, Apr. 2003. ilus
Article
in English
| LILACS
| ID: lil-340116
ABSTRACT
The use of in situ techniques to detect DNA and RNA sequences has proven to be an invaluable technique with paraffin-embedded tissue. Advances in non-radioactive detection systems have further made these procedures shorter and safer. We report the detection of Trypanosoma cruzi, the causative agent of Chagas disease, via indirect and direct in situ polymerace chain reaction within paraffin-embedded murine cardiac tissue sections. The presence of three T. cruzi specific DNA sequences were evaluated a 122 base pair (bp) sequence localized within the minicircle network, a 188 bp satellite nuclear repetitive sequence and a 177 bp sequence that codes for a flagellar protein. In situ hybridization alone was sensitive enough to detect all three T. cruzi specific DNA sequences
Full text:
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Index:
LILACS (Americas)
Main subject:
Trypanosoma cruzi
/
DNA, Protozoan
/
Heart
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2003
Type:
Article
Affiliation country:
Brazil
/
United States
Institution/Affiliation country:
The Medical College of Georgia/US
/
Universidade Federal do Espírito Santo/BR
/
Vanderbilt University/US
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