A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)
Mem. Inst. Oswaldo Cruz
;
98(3): 387-390, Apr. 2003. ilus
Article
in English
| LILACS
| ID: lil-340120
ABSTRACT
Polytene chromosome preparations were obtained from larval, pupal and adult female Malpighian tubules of Aedes aegypti. The Malpighian tubules of the pupae (0-4 h old) from larvae reared at 20ºC provided the best cytogenetic analysis. The interaction of nucleic acids and proteins that influence the spreading of the chromosomes could be reduced with the preparation technique of the sheets submitted to a stronger treatment starting with the hypotony of tissue and successive bathings with acetic acid. A simple technique should facilitate molecular cytogenetics used in the location of resistance and vector competence genes
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Index:
LILACS (Americas)
Main subject:
Chromosome Mapping
/
Aedes
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2003
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual de Campinas/BR
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