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Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
Rubiano, Claudia C; Wasserman, Moises.
  • Rubiano, Claudia C; Universidad Nacional de Colombia. Facultad de Ciencias. Departamento de Química. Laboratorio de Investigaciones Básicas en Bioquímica. Bogotá. CO
  • Wasserman, Moises; Universidad Nacional de Colombia. Facultad de Ciencias. Departamento de Química. Laboratorio de Investigaciones Básicas en Bioquímica. Bogotá. CO
Mem. Inst. Oswaldo Cruz ; 98(5): 693-695, July 2003. ilus
Article in English | LILACS | ID: lil-344291
ABSTRACT
A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10² to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells
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Full text: Available Index: LILACS (Americas) Main subject: Plasmodium falciparum / Polymerase Chain Reaction / Telomere / Telomerase Type of study: Diagnostic study Limits: Animals Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2003 Type: Article / Project document Affiliation country: Colombia Institution/Affiliation country: Universidad Nacional de Colombia/CO

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Full text: Available Index: LILACS (Americas) Main subject: Plasmodium falciparum / Polymerase Chain Reaction / Telomere / Telomerase Type of study: Diagnostic study Limits: Animals Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2003 Type: Article / Project document Affiliation country: Colombia Institution/Affiliation country: Universidad Nacional de Colombia/CO