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Template preparation for rapid PCR in Colletotrichum lindemuthianum
Roca, M. Gabriela; Davide, Lisete C; Wheals, Alan E.
  • Roca, M. Gabriela; Universidade Federal de Lavras. Departamento de Biologia. Lavras. BR
  • Davide, Lisete C; Universidade Federal de Lavras. Departamento de Biologia. Lavras. BR
  • Wheals, Alan E; University of Bath. Department of Biology and Biochemistry. GB
Braz. j. microbiol ; 34(1): 8-12, Jan.-Apr. 2003. ilus
Article in English | LILACS | ID: lil-344557
RESUMO
Isolation of DNA for PCR is time-consuming and involves many reagents. The aim of this work was to optimise a rapid and easy PCR methodology without previous DNA isolation. Different strains of the phytopathogenic fungus Colletotrichum lindemuthianum were used. Protoplasts were generated using lytic enzymes under high incubation temperatures using different methodologies to obtain the template. A rapid (10 minute) methodology was successful for smaller amplicons (<750 bp).
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Full text: Available Index: LILACS (Americas) Main subject: In Vitro Techniques / Polymerase Chain Reaction / Colletotrichum Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2003 Type: Article Affiliation country: Brazil / United kingdom Institution/Affiliation country: Universidade Federal de Lavras/BR / University of Bath/GB

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Full text: Available Index: LILACS (Americas) Main subject: In Vitro Techniques / Polymerase Chain Reaction / Colletotrichum Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2003 Type: Article Affiliation country: Brazil / United kingdom Institution/Affiliation country: Universidade Federal de Lavras/BR / University of Bath/GB