Production of hydrolytic enzymes by Trichoderma isolates with antagonistic activity against Crinipellis perniciosa, the causal agent of witches' broom of cocoa
Braz. j. microbiol
;
34(1): 33-38, Jan.-Apr. 2003. graf
Article
in English
| LILACS
| ID: lil-344562
ABSTRACT
Two isolates of Trichoderma, which reduce the incidence of witches'broom disease caused in cocoa by Crinipellis perniciosa, were evaluated for their potential to produce hydrolases in liquid medium. Very low or no hydrolytic activity was produced in the absence of any substrate. The activities of chitinase, N-acetylglucosaminidase, beta-1,3-glucanase, total cellulase, endoglucanase, aryl- beta-glucosidase, beta-glucosidase, protease and amylase increased dramatically within 72-120 h of growth in the presence of specific substrates. Except for N-acetylglucosaminidase and beta-glucosidase Trichoderma harzianum isolate 1051 produced the largest amounts of hydrolases. The possible involvement of these enzymes in the antagonistic interaction between Trichoderma and C. perniciosa is discussed.
Full text:
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Index:
LILACS (Americas)
Main subject:
Acetyl-CoA Hydrolase
/
Plant Diseases
/
Trichoderma
/
In Vitro Techniques
/
Genes, Fungal
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2003
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Empresa Brasileira de Pesquisa Agropecuária/BR
/
Universidade de Brasília/BR
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