PCR detection of four periodontopathogens from subgingival clinical samples
Braz. j. microbiol
;
34(1): 81-84, Jan.-Apr. 2003. tab
Article
in English
| LILACS
| ID: lil-344571
RESUMO
In this study, A. actinomycetemcomitans, B. forsythus, P. gingivalis and F. nucleatum were identified from subgingival plaque from 50 periodontal patients and 50 healthy subjects. Subgingival clinical samples were collected with sterilized paper points and transported in VMGA III. From all the diluted clinical samples (110), DNA was obtained by boiling, and after centrifugation the supernatant was used as template. Specific primers for each bacterial species were used in PCR. PCR amplification was sensitive to identify these organisms. PCR products from each species showed a single band and can be used to identify periodontal organisms from clinical specimens. PCR detection odds ratio values for A. actinomycetemcomitans and B. forsythus were significantly associated with disease showing a higher OR values for B. forsythus (2.97, 95 percent CI 1.88 - 4.70). These results suggest a strong association among the studied species and the periodontal lesion.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Periodontal Diseases
/
In Vitro Techniques
/
Polymerase Chain Reaction
/
Blood-Borne Pathogens
Type of study:
Diagnostic study
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2003
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade de São Paulo/BR
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