Age-related biological activity of south american rattlesnake (Crotalus durissus terrificus) venom
J. venom. anim. toxins incl. trop. dis
;
9(2): 186-201, 2003. ilus, tab, graf
Article
in English
| LILACS
| ID: lil-345737
ABSTRACT
An in vitro and in vivo comparative study was performed on the effects of Crotalus durissus terrificus venoms from a mother and its 15 newborns. The venoms were tested for protein content, lethality, proteolytic, myotoxic, hemorrhagic, and phospholipase A2 activity. The minimum coagulant dose in plasma and human fibrinogen, protrhombin, and Factor II activations were analyzed. The venoms were also analyzed by polyacrylamide gel electrophoresis (PAGE). This showed that despite similar total protein content, the biological effects of the venoms were different. Venom from young snakes exhibited higher enzymatic and coagulant activities and higher myotoxicity compared to the mother's. In addition, the PLA2 content paralleled myotoxicity. However, no difference could be detected in their toxicity (LD50 0.08 mg/Kg). High incidence of blood coagulation disorders and elevated circulating myoglobin may characterize systemic envenoming by young C. d. terrificus.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Blood Coagulation
/
Crotalus
/
Crotalid Venoms
Limits:
Animals
Country/Region as subject:
South America
Language:
English
Journal:
J. venom. anim. toxins incl. trop. dis
Journal subject:
Toxicology
Year:
2003
Type:
Article
Affiliation country:
Brazil
/
United kingdom
Institution/Affiliation country:
Intituto Butantan/BR
/
Universidade do Amazonas/BR
/
University of Liverpool/GB
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